LED red (R) and blue (B) continuous light (CL) is a potential efficient way to increaseplant productivity of plant factory with artificial light (PFAL), but limited information wasexplored about their effects on plant mineral nutrition. In an environmentally controlled plantfactory with artificial light (PFAL), the effects of CL of different intensities and spectral qualities,emitted by R and B LEDs on growth and nutrient element content and accumulation of lettuce(Lactuca sativa L.), were conducted in three hydroponic experiments. Two treatments, normal light(12 h/12 h) and CL (24 h/0 h) in experiment 1, three CL intensities (100, 200 and 300 μmol∙m−2∙s−1) inexperiment 2, and three CL light qualities (1R:3B, 1R:1B and 3R:1B) in experiment 3 weredesigned. The results showed that CL significantly increased the fresh and dry lettuce shootbiomass compared with normal light, and shoot fresh and dry biomass increased with theintensity increment of CL. In experiment 3, shoot fresh biomass was great under high R lightproportion CL treatment, while dry shoot biomass remained unchanged. Both CL and CL withincreased intensities promoted shoot C content and accumulation in lettuce. CL reduced N, P, K,Ca, Mg, Cu and Zn contents in lettuce shoot, while Fe and Mn contents did not change comparedto NL. Moreover, CL increased Ca, Fe and Mn accumulation.